H2N2V1, Main, Exploration, bibRecord, 001699

Cell-based assay for the determination of temperature sensitive and cold adapted phenotypes of influenza viruses

Identifieur interne : 001699 ( Main/Exploration ); précédent : 001698; suivant : 001700

Cell-based assay for the determination of temperature sensitive and cold adapted phenotypes of influenza viruses

Auteurs : I. Kiseleva [États-Unis, Russie] ; Q. Su [États-Unis] ; T. J. Toner [États-Unis] ; C. Szymkowiak [États-Unis] ; W.-S. Kwan [États-Unis] ; L. Rudenko [Russie] ; A. R. Shaw [États-Unis] ; R. Youil [États-Unis]

Source :

Journal of virological methods [ 0166-0934 ] ; 2004.

RBID : Pascal:04-0395291

Descripteurs français

KwdFr :
- Animaux, Basse température, Chiens, Embryon de poulet, Lignée cellulaire, Phénotype, Réplication virale, Température, Vaccins antigrippaux, Vaccins atténués, Virus de la grippe A (génétique), Virus de la grippe A (pathogénicité), Virus de la grippe A (physiologie), Virus influenza B (pathogénicité), Virus influenza B (physiologie), Virus recombinants (génétique), Virus recombinants (physiologie).
MESH :
- génétique : Virus de la grippe A, Virus recombinants.
- pathogénicité : Virus de la grippe A, Virus influenza B.
- physiologie : Virus de la grippe A, Virus influenza B, Virus recombinants.
Pascal (Inist)
- Animaux, Basse température, Chiens, Embryon de poulet, Influenzavirus, Lignée cellulaire, Phénotype, Réplication virale, Température, Microbiologie, Méthode, Vaccins antigrippaux, Vaccins atténués, Virologie, Grippe.

English descriptors

KwdEn :
- Animals, Cell Line, Chick Embryo, Cold Temperature, Dogs, Influenza, Influenza A virus (genetics), Influenza A virus (pathogenicity), Influenza A virus (physiology), Influenza B virus (pathogenicity), Influenza B virus (physiology), Influenza Vaccines, Influenzavirus, Method, Microbiology, Phenotype, Reassortant Viruses (genetics), Reassortant Viruses (physiology), Temperature, Vaccines, Attenuated, Virology, Virus Replication.
MESH :
- chemical : Influenza Vaccines, Vaccines, Attenuated.
- genetics : Influenza A virus, Reassortant Viruses.
- pathogenicity : Influenza A virus, Influenza B virus.
- physiology : Influenza A virus, Influenza B virus, Reassortant Viruses.
- Animals, Cell Line, Chick Embryo, Cold Temperature, Dogs, Phenotype, Temperature, Virus Replication.

Abstract

The determination of temperature sensitive (ts) and cold adapted (ca) phenotype for influenza A and B strains has been conducted traditionally using embryonated chicken eggs. As attempts are made to move away from the use of eggs in the manufacturing process of influenza vaccines, it will become useful to develop cell-based assays to support cell culture-based vaccine production. In this study, MDCK cells have been evaluated as a tool for determining the ts and ca phenotypes associated with live attenuated influenza viruses. Direct comparisons were made of these phenotypes carried out in eggs. Reassortants made from the Russian live attenuated influenza donor strains A/Leningrad/134/17/57 (H2N2) and B/USSR/60/69 were prepared entirely in MDCK cells and their phenotypes evaluated using the MDCK cell-based assay. It is concluded that MDCK cells are more sensitive than eggs for the measurement of is and ca phenotype of influenza viruses (particularly for influenza A) and they provide an alternative means for screening candidate reassortants prior to determining their genome composition.

Affiliations:

Le document en format XML

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<term>Cell Line</term>
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<front><div type="abstract" xml:lang="en">The determination of temperature sensitive (ts) and cold adapted (ca) phenotype for influenza A and B strains has been conducted traditionally using embryonated chicken eggs. As attempts are made to move away from the use of eggs in the manufacturing process of influenza vaccines, it will become useful to develop cell-based assays to support cell culture-based vaccine production. In this study, MDCK cells have been evaluated as a tool for determining the ts and ca phenotypes associated with live attenuated influenza viruses. Direct comparisons were made of these phenotypes carried out in eggs. Reassortants made from the Russian live attenuated influenza donor strains A/Leningrad/134/17/57 (H2N2) and B/USSR/60/69 were prepared entirely in MDCK cells and their phenotypes evaluated using the MDCK cell-based assay. It is concluded that MDCK cells are more sensitive than eggs for the measurement of is and ca phenotype of influenza viruses (particularly for influenza A) and they provide an alternative means for screening candidate reassortants prior to determining their genome composition.</div>
</front>
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Cell-based assay for the determination of temperature sensitive and cold adapted phenotypes of influenza viruses

Cell-based assay for the determination of temperature sensitive and cold adapted phenotypes of influenza viruses

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